November 10, 2024
Simplified Measurement of Any DNA Methyltransferase
DNA methylation is controlled at several different levels in normal and diseased cells. The addition of methyl groups is carried out by a family of enzymes, DNA methyltransferases (DNMTs). Three DNMTs (DNMT1, DNMT3A, and DNMT3B) are required for the establishment and maintenance of DNA methylation patterns. The importance of DNA methylation is emphasized by the growing number of human diseases that are known to occur when DNA methylation information is not properly established and/or maintained. A researcher can now individually measure each of the amounts of DNMTs, 1, 3A, and 3B, or measure total DNMT activity/inhibition using an ultra-sensitive and highly specific ELISA-based assay which can be completed in as little as 4 hours.
Optimized DNA Methylation & Hydroxymethylation Quantification
Due to the presence of both 5-mC and 5-hmC in DNA, which may have different functions, it is important to determine the quantities of these two modified nucleotides and their ratios in different cell types and in different compartments of the genome. It is particularly important to identify whether epigenetic change at the DNA level is due to methylation or hydroxymethylation in healthy and diseased human cells/tissues. A researcher can overcome cumbersome and time-consuming 5-mC and 5-hmC detection methods through the use of an optimized ELISA-like kit which utilizes an innovative kit composition that enables background signals to be extremely low and can be completed within just 4 hours.
MeDIP-grade 5-Methylcytosine Antibody
Epigentek’s mouse monoclonal antibody from clone 33D3 is highly-specific to 5-methylcytosine, with no cross reaction to other modified cytosines. This high quality antibody has been extensively tested for its specificity in various applications including ELISA, immunohistochemistry, immunofluorescence, and MeDIP.